This time, we will have not one, but two exciting presentations on different super-resolution and label-free imaging technologies! Therefore please reserve 1 hour on Friday 24th November 2023 to join us in the 9th Tech Exchange episode of 2023!
Registration link below.
Øystein Ivar Helle and Merete Storflor from Chip NanoImaging will talk about “Ultra-large field of view in both super-resolution and diffraction-limited mode, using photonic chips”.
Chip-based TIRF offers an ultra-large field of view (FOV), capturing the diversity occurring in biological processes. Cells are cultured directly on a photonic chip and can be imaged either in a live or fixed state, tissue samples will also adhere to the chip surface. The photonic chip has integrated multimode waveguides that generate a well-defined evanescent field at the interface between the sample and the waveguide. The homogeneous illumination traverses the entire waveguide, resulting in precise optical sectioning that is particularly well-suited for exploring membrane dynamics.
The tight light confinement and strong evanescent field is ideal for super-resolution techniques such as fluorescence fluctuation-based microscopy (FF-SRM), and single-molecule localization microscopy (SMLM) providing enhanced contrast and resolution across a millimeter-scale FOV. The large datasets are efficiently reconstructed with user-friendly GPU-accelerated post-processing software. Chip NanoImaging provides simplified, uniform, multi-channel TIRF illumination, that not only reduces demands on the end-user’s expertise but also significantly extends the field of view.
The second talk by Michal Kotek, ” Telight – Exploring the unseen”, will introduce our latest Industry Board member, Telight.
Telight specializes in the development of light optics instruments for life sciences. It offers innovative solutions for research centers, laboratories, and universities worldwide. Their focus is on the development of highly specialized devices for live-cell analysis. An example of development in Brno is a holographic microscope Q-Phase, which won the Werner von Siemens Award for the most critical development and innovation in 2013. It is used for a variety of research applications, such as the study of migrastatics, new substances proved to help with curing cancer.