The Euro-BioImaging Industry Board has organized an exclusive 1-day workshop for its members and staff from the Euro-BioImaging Nodes on the subject of “Smart Microscopy” at the Imaging Centre at EMBL, Heidelberg, on Thursday13th October 2022, which was attended by 87 on-site and online participants.
Smart microscopy connects bioimage analysis with computer-controlled microscopy to generate automated and adaptive imaging workflows to enable quantitative, statistically meaningful results in complex biological systems. This approach brings new challenges, such as accessing and controlling instrument hardware via suitable software, designing good workflows or handling of large, terabyte-sized data sets during operation.
The workshop managed to bring together leading instrument and software providers with experts from our Euro-BioImaging Nodes for fruitful discussions around research needs, technical requirements and existing solutions for Smart Microscopy.
Recordings of the Keynote lectures are available on our YouTube Channel.
The workshop participants concluded that the first steps towards standardization and making Smart Microscopy more accessible would be to
- Build a consensus on what the field should be called and what is included
- Agree on a standard ontology for Smart Microscopy and workflow components to be used and adopted across different instrument control and analysis software and define a list of core workflow components that should be accessible in each software
- Identify the top community needs of microscope users and facilities for smart microscopy (from data format or interoperability issues to technical access requirements)
We would like to discuss this further with the community and collect YOUR input for further discussion with instrument and software producers.
We are looking forward to hearing from you about your opinion on standards and best practices in this emerging field!
Agenda
| Time | Topic |
|---|---|
| 10:00 – 10:10 | Martin Tewinkel, Evident Olympus and Board Chair Welcome and short intro Euro-BioImaging Industry Board |
| 10:10 – 10:15 | Claudia Pfander, Board Coordinator Meeting agenda and objectives |
| 10:15 – 10:45 | Rafael Camacho, University of Gothenburg Good workflow design - Introduction and Keynote |
| 10:45 - 11:00 | Marc Tramier, MRic facility CNRS/ University of Rennes, France Bio-Imaging Node The roboscope: smart automated microscopy using real time embedded artificial intelligence combining microscopy control |
| 11:00 - 11:15 | Evangelis Sisamakis, PicoQuant GmbH FLIM, FCS and single molecule FRET workflows in Luminosa, the new single photon counting confocal microscope by PicoQuant |
| 11:15 - 11:30 | Laurent Thomas, Acquifer High-throughput imaging with the ACQUIFER Imaging Machine: versatile scripting opportunities |
| 11:30 - 11:45 | Christoph Greb, Leica Microsystems AI-based ROI Definition for Laser Microdissection |
| 11:45 - 12:30 | Discussion round |
| Break | |
| 13:30 - 14:00 | Aliaksandr Halavatyi, EMBL Tools and software interfaces - Introduction and keynote |
| 14:00 - 14:15 | Ludovico Silvestri, European Laboratory for Non-linear Spectroscopy Online correction of sample-induced defocus in light-sheet fluorescence microscopy |
| 14:15 - 14:30 | Marco Castello, Genoa Instruments - Istituto Italiano di Tecnologia Carma: an FPGA-based microscopy platform for (image) scanning microscopy |
| 14:30 - 14:45 | Giacomo Mazzamuto, National Research Council - National Institute of Optics (CNR-INO) and LENS Tools and strategies for high-throughput light-sheet Fluorescence Microscopy |
| 14:45 - 15:30 | Discussion round |
| Break | |
| 16:15 - 16:45 | Antonio Politi, MPI Göttingen Feedback microscopy and data analysis - Introduction and keynote |
| 16:45 - 17:00 | Kees Jalink, Netherlands Cancer Institute (Euro-BioImaging Functional Imaging Node) Adapting high-content microscopy screening to study dynamic signaling mechanisms |
| 16:00 - 17:15 | Manoel Veiga Gutierrez, EVIDENT Technology Center Europe GmbH Workflow for analyzing high-content microscopic image data |
| 17:15 - 17:30 | Roman Zinner, Carl Zeiss Microscopy GmbH Data Storage and Processing Server |
| 17:30 - 17:45 | Alberto Diaspro, UNIGE-IIT The intelligent microscope |
| 17:45 - 18:30 | Discussion round |